DNA Extraction Lab Report Essay Example

DNA Extraction Lab Report Paper Extraction and purification of DNA are the first steps in the analysis and manipulation of DNA that allow scientists to detect genetic disorders, produce DNA finger rings of individuals, and even create genetically engineered organisms that c an produce beneficial products such insulin, antibiotics, and hormones. DON A can be extracted from many types of cells. For this lab, we extracted DNA from a strawberry and our own cheek cells. D AN can be extracted from cells by a simple technique with household chemicals, enable Eng us to see strands of DNA with the naked eye. In this lab, we added a buffer solution composed of salt, clear dish detergent, and denizen d water for procedure the strawberry part). For procedure 2( our cheek cells), e used the same buffer solution but added red food coloring so we can dusting sushi the DNA from the rest of the cellular components because it was all a white mixture. Finally, ethanol was added in the test tubes because DNA is soluble in w eater and alcohol causes DNA to isolate from the solution, leaving behind all the c alular components that arent soluble in alcohol. These solutions allow the break kiddo of lipids surrounding the DNA such as the nuclear envelope and proteins that make up a cell membrane. A few minutes later, clumps of a white substances e that looks like mucus can be seen. That mixture of DNA an be spooled with a stirring rod and pulled out of the solution. We will write a custom essay sample on DNA Extraction Lab Report specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on DNA Extraction Lab Report specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on DNA Extraction Lab Report specifically for you FOR ONLY $16.38 $13.9/page Hire Writer Design for procedure 1: Hypothesis when DNA extraction buffer is added to a strawberry mixture the DNA becomes visible. Dependent variable: DNA in strawberry Independent variable: DNA extraction buffer Control group: Strawberry mixture in the bag without the buffer Experimental group: strawberry mixture with DNA extraction buffer Design for procedure 2: Hypothesis when you gargle salt water your cheek cells get suspended in the salt water after being spit out. Dependent: cheek cells Independent: salt water Control group: regular water Experimental: salt water Materials for 1st Procedure: DNA extraction buffer (900 ml of denizen water, 50 ml of CLC AR dishwashing detergent, 2 teaspoons of salt), a whole straw Webber, suspicious bag, plastic cups, cheesecloth, funnel, test tubes, pipette, paper towel, stirring rod, and ethanol Materials for 2nd procedure: DNA extraction buffer (900 ml of denizen water, 50 ml of CLC ear dishwashing detergent, 2 teaspoons of salt), a whole straw Webber, suspicious bag, plastic CUPS, cheesecloth, funnel, test tubes, pipette, paper towel, stirring rod, red food coloring, and ethanol Method for procedure 1: We began this part of the lab by adding a strawberry to a Copilot storage back and one of our group members used her hands to smash the strawberry. W e then added 10 ml of the DNA extraction buffer. We mashed the strawberry in the buffer for one minute. Then, we used a funnel and cheesecloth (or coffee e filters) to filter the strawberry juice into a single test tube or beaker. After the at, we slowly poured cold ethanol or 91% spoilsport alcohol over the top o f the strawberry mixture. To get a single layer of clear ethanol on top of the strawberry mixture, we used a pipette to add the ethanol. When we observed who tie strands forming in the ethanol layer, we used an inoculating loop or stirring rod to spool the strands. Method for procedure 2: We first select a group member to participate in the experiment. We obtained a cup and poured 200 MI of drinking water and added two teaspoons of salt . He (Frank) gargled the salt water for 1 minute. After the minute was up, he spit the gargled water into another cup. By doing this, his cheek cells were e now suspended in the salt water. He gently stirred the salt water with o en drop of soap. We then mixed 20 ml spoilsport alcohol and 13 drops of DOD coloring in a separate beaker. Then, we transferred the gargled salt water/ soap solution to a test tube to make it easier to see the DNA. After, we gently pop rued the colored alcohol over the top of the gargled water and it formed a layer on top. We waited for about 2. 5 minutes and we saw white clumps and strings forming. THIS WAS DNA! FRR Ann. Then used a stirring rod to spool the clumps of DNA out of the solution. Analysis What does DNA look like? The DNA is in the form of a spider web. Why is it important for scientists to be able to remove DNA from cells? It is important because DNA shows the genetic makeup of organisms and are the building blocks of differentiation between organisms. What is the role of detergent, ethanol, and salt in the extraction process? Deters .NET allows the breakdown of the nuclear membrane so DNA can come out. Ethan 01 allows DNA to clump up so it can be extracted much easier. Salt helps put and keep the DNA together. Is there DNA in your food? How do you know? Why are you not harmed (or altered) by ingesting the DNA of another organism? What implication does this have for farmers who would use GM (genetically modified) plants? Yes, there is DNA in our food; the strawberry DNA extraction experiment proved this to be true. We are not harmed by the DNA because they cant survive the internal conditions of our body. Once they enter the acidic environment of our stomachs, they are broken down and they can no longer function properly or at all; they are denatured. Therefore, they are not able to harm us. Its implication is that they can continue using it since it doesnt harm our cells. Conclusion To recap, DNA is a small molecule composed of four nucleotide bases; Adenine, Thymine, Guanine and Cytosine, Guanine pairing with Cytosine, and Thymine pap inning with Adenine. The DNA is in the shape of double helix strands. DNA is the bal preprint to life; every living thing contains DNA From this lab, our group has learned how to extract DNA from strawberries and ourselves and have it made visible to the naked eye. Strawberries have eight sets of chromosomes. For procedure 1 , the goal is to e extract DNA from the strawberry. The purpose of the lab is to extract the DNA from the cells and observe it visibly with the human eye. In order to extract DNA from them, you must simply take the pink strawberry solution. By using ethanol, detergent, and salt, you will e that DNA will soon become visible, making it appear as a white mucus or in a spider formation. Thats how you can see it is ready for access able extraction. For procedure 2, the same buffer is used to extra CT DNA from ones cheek cells, but is dyed red to distinguish the DON A from other components. The solutions used for both procedures break down substances surrounding the DNA , such as the nuclear envelope, proteins, and other components. Once clumps of a white substance ( DNA) become visible, they can be pulled out of the test TU be with a stirring rod and examined without a microscope!

Molly Maguires essays

Molly Maguires essays The Molly Maguires also referred to, as the Mollies or the sons of Molly Maguire, is not a single person who influenced the birth of the Union, but a group of Irish immigrants who created a lot of controversy. Over all, there have been differing views on the Molly Maguires. Some would say what they did was reprehensible and vicious while, others might contend they were heroes in their time, and did only what they had to do to fight for better working conditions. Regardless of what side of the argument you favor, one thing is sure, they had enough influence to start a new era for working Unions. Since the Mollies went about getting what they thought was right and fair rather cruelly, it is obvious they felt the ends justified the means. In 1700 Ireland, many of the English landlords were brutal to their tenants, by killing them or setting fire to their homes. When they set fires, it was to run a tenant out so that they could raise the rent on the new renter. The Mollies were said to have taken their name from an elderly widow named Molly Maguire who was driven out of her home by fire. The men who came to her rescue decided to take revenge. They called themselves The Sons of Molly Maguire, and would dress up as women, then rob and kill the evil landlords. In Pennsylvania, where the most infamous Mollie acts occurred, the Irish endured much the same things as in Ireland. In the 1860s, Irish immigrants faced similar discrimination many African-Americans endure today. Jobs were not easily found, but if someone was hiring, their help wanted sign usually had Irish Need Not Apply at the bottom. The Irish coalminers were treated like slaves. Although they did receive wages for their work, everything was deducted from their wages. One could not purchase products in any other store than the town store; which was owned and operated by the mine own...

The use of prophylactic antibiotics to reduce the instances of Research Paper

The use of prophylactic antibiotics to reduce the instances of surgical site infections - Research Paper Example We will write a plan for the implementation and evaluation of this plan and its application guidelines. In the interest of covering the trend of electronic health records, the plan will involve the use of newer technologies in maintaining a 100% antibiotic prophylaxis rate. While much has been written concerning which medications to use, when and how, I will take the Stetler Model to use newer technologies to improve upon current problems and provide new ideas to that extent. Phase 1: Introduction and Preparation A great many strides have been made since the advent of antibiotics that have helped cure infection and even prevent them from happening. In surgical care, because the body is being necessarily invaded normal flora can be translated to areas in which they become pathogenic and cause infection and disease (Wells, 2009) . Surgical site infections occur as a result of these bacteria and cause disease in the following manners: Incisional Deep (muscle and fascial layers) Subcutan eous (cellulitis) Organ or Space (Abscess or Meningitis) Further, research conducted by White (2007) shows how despite national recommendation guidelines many hospital surgical teams fail in achieving a 95%+ rate of surgical antibiotic prophylaxis to prevent post-operative infections which can occur as late as 30 days after the procedure (Wells, 2009). The most common soft tissue infectionsare gram positive organisms and are easily treated or prevented, but if allowed to infect areas of the body without prior treatment they can reproduce and cause abscesses, then the chance of damaging infections where organ function is compromised can be adequately controlled using the Stetler model to be implemented here. Appendix 1 will show the replication of the National Research Council Wound Classification. Interest Interest in this topic stems from insight to the needs we have at present to improve some of the antiquated paper systems in place with one that provides for the needs of the pati ent population, accounting for human error, that will be incurring a surgical procedure and that would be all patients from pediatric to geriatric. The need to limit infection as a complication of an already difficult medical practice is tantamount to improving post-operative infection outcomes where co-morbidities are not common place, but rare and limited. Patient Population and Clinical Settings As mentioned, almost every patient will at one time need a surgical procedure. However, each operation is different and carries risks unique to itself. A colectomy will certainly need different antibiotic treatment than a simple cyst removal or even a transplant operation, however the pre-surgical needs are all the same. Each patient needs to meet with the anesthesiologist, the operating surgeon if they haven't prior to the date, and nursing staff who will be caring for them throughout the process. In each, be it an outpatient facility, hospital, or clinic, appropriate treatments should b e administered and proper documentation followed. Validation and Literature Review A complete and thorough review of current literature regarding surgical prophylaxis was performed using Google Scholar for literature review, PubMed, and text sources. Search target parameters included 'surgical prophylaxis error', 'nursing surgery error', 'pre-surgical antibiotics' and 'surgical infection'. After a thorough review,

It's the Law ... or is it Essay Example | Topics and Well Written Essays - 500 words

It's the Law ... or is it - Essay Example There exist set rules and laws that are useful in curbing the misuse of other people’s work found in the web. The paper will briefly explore copyright laws governing internet information and the penalties for contravening them. There are numerous laws and regulations governing the use of internet information, but the paper will particularly look at copyright laws. In 1998, the then United States president Bill Clinton signed the digital millennium copyright Act (Scott). The act provides guidelines that should be adhered to by the users of the internet when accessing other people’s work that may include, music and intellectual work among others. Further, Scott documents that some of the copyright laws and regulations include: fair use, unauthorized access and copying, file sharing and liability. Let us examine liability. As stipulated in the copyright Act, one is not allowed to produce and distribute unauthorized copies from the internet. In the event that producti on is to be done, consent of the producer is supposed to be sought. Failure to do the same amounts to a breach of the law and the offender is liable for the legal consequences. When the breach occurs, the plaintiff can sue the actual offender or the internet service provider in a court of law. The complainant is also at liberty to seek an injunction from the court (Lesley). The purpose of the injunction is to prevent the offender from continual violation of the copyright rights. Lesley further documents that the copyright holder has the right to seek compensation from the offender in the court in form of monetary or statutory damages. The court of law is charged with the responsibility of establishing the guilt or innocence of the accused. If one is found guilty of violation the copyright act, he or she must take criminal responsibility.

Sport Effect on Teens Essay Example for Free

Sport Effect on Teens Essay Sports serve more than just a physical purpose such as teaching teamwork. Even individual sports such as wrestling, foster team environment in which teens can challenge themselves supports each other and cheer each other. Sports are alsoprovides additional role models and positive adult influence in the teens lives through coaches and the athletic administration staffs. The more adults a teen has to turn to, the more likely he is to go to someone when he faces challenges. Sports are away kids get introduced to different physical activities. Sports help kid learn skills, including how to dribble a basketball, kick a soccer ball or leap over a hurdle. It takes discipline and hard work. Also, involvements in sports can introduce your kids a new group of kid who have atleast one common interest. Sports require a lot of Teens time as most teams practice or have competitions several days per week during season she isn’t practicing or competing, most likely studying to stay eligible, eating or sleeping. Teens who participate in sports through their school are governed by state rules and regulations, as well as school rules they must follow eligible. This typically consists of a maximum grade point average, not failing any classes and being held to a higher standard for citizenship with in their school and community . Students tend to work harder to maintain eligibility when playing sports, which result in accountability and pride. Teens also, who participate in sports have fewer drop out of pregnancies. The reason are debatable, but most people feel that being a part of a team and activities keep teens out of trouble because they want to be a part of a group, any group ,and the old saying that idle hands are the devils instruments are at least partially true. Another reason why does participation in sports keep teens out of trouble because teens have too much fun doing their favorite sport to be causing trouble . They’re interested in if they are going to win or not if the coach is going to make them captain . Whatever it is, they are more worried about their sports and having fun rather than doing dumb stuff and worrying their parents. Sport also keep teens out of trouble because it’s when they’re bored out of their minds, that they look into other avenues for excitement . They find there is nothing afire publicly. So they go for the thrill of what has never been tried before. You and I both know it all been tried but they think they can do it. In the end they can’t get away with it. It’s all been done and it can’t be done legally. Participation in sports can help keep teens out of trouble, though it’s not guaranteed. Teens who participate in sports tend to have less time to get into trouble. In better physical shape than their sedentary peers, they tend to pay more attention to nutrition than kids who don’t play sports. Teen athletes also tend to do better academically and may get a boost in self-esteem compared to their peers who do not participate in sports. Participating in sports is good it can enhance our skills . Don’t ignore when it comes to sports because it’s useful for us and we can enjoy our life even though we can cause a trouble and Sports can save you from a life of crime or a life on the streets. Just by being part of something that’s bigger than you can help you change your life around for the better. Being part of a team is like being part of a family. You watch out for one another and support one another. If you’re not playing sports, you may want to consider it. Unfortunately, most schools are cutting extracurricular activities such as football, volleyball, baseball, track, softball, rugby, archery, basketball, and other sports programs. Playing sports is good for your mind, body, and soul. You’ll get fresh air if you play an outdoor sport. You’ll strengthen your bones and muscles. Your mind will be getting exercise as well because you’ll have to remember game plays and terminology. Your soul will be cleansed because you’ll be doing something you love and be part of a team. It’s all good! In conclusion, I agree that participating in sports keep teens out of trouble first they want to be a part of a group, any group ,and the old saying that idle hands are the devils instruments are at least partially true then teens have too much fun doing their favorite sport to be causing trouble. Games can cause trouble but be sports. Everything happens for a reason. I’m used to it, I prepare for it. Like I say, at the end of the day, those in charge of their own destiny are going to do what’s rights for them and their family.

How did Brecht make use of character and audience in order to successfu

Bertolt Brecht was a renowned German theatre practitioner and dramatist whose works are credited as having been at the leading edge of 20th century theatre. Being a socialist-Marxist, Brecht he was deeply concerned with the society in which he lived, and so desired to change the way people both thought and acted towards their fellow man. His concept of the Epic Theatre sought to dramatically change the way in which theatre is to be performed, opposing many of the 19th century dramatic conventions outlined for the ‘well-made play’. Dramatic Theatre is generally thought to be constructed of a number of things; including the use of detailed characters, containing a robust plot, having evolutionary determinism, and progressing with linear development. On the other hand, Brecht’s Epic Theatre focuses on narrative, episodic scenes, curved storylines, and creates a broader picture of the world for the spectator. The primary intention of a ‘well-made play’ is to simply entertain, and as Brecht stated, ‘from the first it has been the theatre’s business to entertain people’ (Brecht, 1964, pg. 180). However, Brecht personally believed that theatre should primarily be a place for learning and social correction – using the stage as a political platform to both inform and inspire its audience members. Although this is not to say that Brecht completely dismissed the notion of having theatrical entertainment, since his plays were also filled with music, comedy and lighting. Thus, theatre should not merely try to represent society – it should attempt to shape it too. The main area that I wish to address is how Brecht made use of both character and audience in an attempt to push forward his own political and moral interests. To do this, I will be l... ...23-31, JSTOR [Online]. Available at: http://www.jstor.org/stable/392365 (Accessed 19/04/2012). Ernst, R. (2005) The Spectator and Not the Actor is the central focus of Brecht’s Stagecraft. Munich: GRIN Publishing GmbH. Kolbe, J. (1954) Portrà ¤t Bertolt Brecht. [electronic print] Available at: http://en.wikipedia.org/wiki/File:Bertolt-Brecht.jpg (Accessed 22/04/2012). Morley, M. (1977) Brecht: A Study. New Jersey: Rowman and Littlefield. Needle, J. (1981) Brecht / Jan Needle and Peter Thomson. Oxford: Blackwell. Styan, J. L. (1981) Modern Drama in Theory and Practice. Cambridge: Cambridge University Press. Tatlow, A. and Wong, T. (1982) Brecht and East Asian Theatre: The Proceedings of a Conference on Brecht in East Asian Theatre. Hong Kong: Hong Kong University Press. Willett, J. (1959) The Theatre of Bertolt Brecht, A Study from Eight Aspects. London: Methuen. How did Brecht make use of character and audience in order to successfu Bertolt Brecht was a renowned German theatre practitioner and dramatist whose works are credited as having been at the leading edge of 20th century theatre. Being a socialist-Marxist, Brecht he was deeply concerned with the society in which he lived, and so desired to change the way people both thought and acted towards their fellow man. His concept of the Epic Theatre sought to dramatically change the way in which theatre is to be performed, opposing many of the 19th century dramatic conventions outlined for the ‘well-made play’. Dramatic Theatre is generally thought to be constructed of a number of things; including the use of detailed characters, containing a robust plot, having evolutionary determinism, and progressing with linear development. On the other hand, Brecht’s Epic Theatre focuses on narrative, episodic scenes, curved storylines, and creates a broader picture of the world for the spectator. The primary intention of a ‘well-made play’ is to simply entertain, and as Brecht stated, ‘from the first it has been the theatre’s business to entertain people’ (Brecht, 1964, pg. 180). However, Brecht personally believed that theatre should primarily be a place for learning and social correction – using the stage as a political platform to both inform and inspire its audience members. Although this is not to say that Brecht completely dismissed the notion of having theatrical entertainment, since his plays were also filled with music, comedy and lighting. Thus, theatre should not merely try to represent society – it should attempt to shape it too. The main area that I wish to address is how Brecht made use of both character and audience in an attempt to push forward his own political and moral interests. To do this, I will be l... ...23-31, JSTOR [Online]. Available at: http://www.jstor.org/stable/392365 (Accessed 19/04/2012). Ernst, R. (2005) The Spectator and Not the Actor is the central focus of Brecht’s Stagecraft. Munich: GRIN Publishing GmbH. Kolbe, J. (1954) Portrà ¤t Bertolt Brecht. [electronic print] Available at: http://en.wikipedia.org/wiki/File:Bertolt-Brecht.jpg (Accessed 22/04/2012). Morley, M. (1977) Brecht: A Study. New Jersey: Rowman and Littlefield. Needle, J. (1981) Brecht / Jan Needle and Peter Thomson. Oxford: Blackwell. Styan, J. L. (1981) Modern Drama in Theory and Practice. Cambridge: Cambridge University Press. Tatlow, A. and Wong, T. (1982) Brecht and East Asian Theatre: The Proceedings of a Conference on Brecht in East Asian Theatre. Hong Kong: Hong Kong University Press. Willett, J. (1959) The Theatre of Bertolt Brecht, A Study from Eight Aspects. London: Methuen.

Want and Marginal Places

The Value of Marginal Places In the essay â€Å"In Praise of Margins† by Ian Frazier, Frazier mentions that the world is a place where adults tend to get caught up in. They do not realize that what they need is that place to breath. He says that marginal places and activities, when he was a kid, were a waste of time, but once he was older and saw his child have their marginal â€Å"moment† playing, in the steam instead of fishing, he realizes the importance as an adult. He mentions that we all have a place as a child that we would like to go back and let go of all our responsibilities.Frazier uses the word margin, this word that Frazier uses, has a negative and positive tone and he explains the time spent in the woods. Frazier states that the definition of margin is a blank space around a body of type or border of a piece of ground. It’s adjective meaning has a negative tone to it; minimal for requirements, almost insufficient. As Frazier uses marginal to describ e what he did in the woods as a child, he grew up to realize how important these â€Å"marginal† places had so much value.As a young boy Frazier, mentions â€Å"the woods† as places where he and his friends would play at all day. Exploring and possibly be lost in the hours of fun they would have ad most importantly, no worries. Exploring things as they go playing along the trees, maybe even being some sort of character or action hero from his time. As Frazier got a little bit older he one day realized that his time spent in the woods was becoming childish and foolish. He eventually stopped going to this place, that as a child was almost like a sanctuary or a place of acceptance to be him-self freely.When Frazier was in the seventh grade he came to asked himself â€Å"What are you doing? † or maybe â€Å"why we are here? † He notices that some of his friends started to not dress appropriately to climb trees and play because of the penny loafers the wore. I am sure this was for the girls they were trying to impress as they get older. As they had other things happening in life that bounded them from going in to the woods, and somehow forgot about it they really never decided to go back and play.But from my understanding, in high school they went back to the woods one day, where they had this sense of ‘’there is nothing here for us anymore† or â€Å"why did we waste our time here as kids†. I am sure high school, girls and school dances had the memories of â€Å"the woods† faded deep in their memory. They realized that they are all grown up and have no need to be there. Frazier must have thought of all the childish senseless things they did being in the woods for hours, just wasting time that they did not know of or care for because they were children.I mean they didn’t have responsibilities as children they didn’t have to work and take of others. I guess that was the good thing about margin al places. For Frazier, when we took his children to go fishing, they sort of did not want to go fishing and instead played in a ditch. Frazier did not have a heart to tell them to stop; instead he realized that this was a marginal place his children will one day grow up turned this ditch in into a marginal place. Just like when Frazier was a child and did not know this was a marginal place he sees that with his kids, and realizes the value of having a marginal getaway.In my opinion, I agree with Frazier’s ideas that â€Å"marginal† activities and places are valuable. Marginal places are also a way of making oneself a better person. It’s a place to think and relax from the worlds’ needs and wants. Of all the places in the world everybody has their own marginal area. Mine so happens to be at Fontainebleau State Park in Mandeville, Louisiana. As a child my parents would pack up the car and head over across the lake every Saturday if the weather permitted.Th is place had a pool, walking trail, barbeque pits and most importantly a beach, not a five star beach but to a 6 year old it was. I would walk along the shore where the water would meet the trees and I would pretend it was undiscovered land. I still go back to this day, monthly and it’s still the same feeling I get, a breath of fresh air. This is what Frazier wants people to know about having marginal places and doing activities, they are not bad places, everyone needs a break from the a world that moves at fast pace.In Conclusion Frazier wants people to know that having a bit of time to youself is okay. Everyone has an escape, whether if it is an old place as a kid, a cartoon you use to watch or even the music you use to listen to. Marginal does not have to be in a negative use. In some cases like Frazier it was a good thing, sort of almost like a re-evaluation of life and I am sure he doesn’t regret playing in the woods as a child at all. Ian Frazier looks to the woo ds as a safe and happy place. I am sure he wants us to remember the marginal place we had as children also.